모든 사진(1)
About This Item
실험식(Hill 표기법):
C25H22N2O5
CAS Number:
Molecular Weight:
430.45
MDL number:
UNSPSC 코드:
12171500
NACRES:
NA.47
추천 제품
제품명
5-Carboxytetramethylrhodamine,
분석
≥95% (HPLC)
Quality Level
양식
solid
solubility
water: slightly soluble
ε (흡광계수)
≥85000-97000 at 539-545 nm in methanol
형광
λex ~543 nm; λem ~570 nm in methanol
응용 분야
diagnostic assay manufacturing
hematology
histology
저장 온도
room temp
SMILES string
N(C)(C)c1cc2[o+]c3c(c(c2cc1)c4c(cc(cc4)C(=O)[O-])C(=O)O)ccc(c3)N(C)C
InChI
1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)
InChI key
YMZMTOFQCVHHFB-UHFFFAOYSA-N
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일반 설명
An important fluorophore prepared in high purtity with a detection limit of TMR-labeled amino acids by capillary electrophoresis of approx. 600 molecules. Readily excited by the 546 nm spectral line from mercury-arc lamps used in fluorescent microscopes, and is intrinsically more photostable than fluorescein.
애플리케이션
5-Carboxytetramethylrhodamine has been used in fluorophore characterization.
생화학적/생리학적 작용
5-Carboxytetramethylrhodamine can be covalently attached to the DNA and is thereby used in studies involving branched and duplex DNA/RNA structures. It is mainly used for single-molecule studies. 5-Carboxytetramethylrhodamine is also used for the labelling of oligosaccharide for laser-induced fluorescence detection.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
이미 열람한 고객
G Vámosi et al.
Biophysical journal, 71(2), 972-994 (1996-08-01)
Fluorescence steady-state and lifetime experiments have been carried out on duplex and single-stranded DNA molecules labeled at the 5' ends with 5-carboxytetramethylrhodamine (TMRh). The temperature and ionic strength of the solutions were varied over large ranges. The results reveal at
Magali Court et al.
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ACS applied materials & interfaces, 9(46), 40059-40069 (2017-10-13)
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PloS one, 8(12), e80390-e80390 (2013-12-07)
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