21819

Carbol-Fuchsin solution according to Kinyoun

• 동의어(들): Kinyoun’s carbol-Fuchsin
• MDL number: MFCD00143924
• UNSPSC 코드: 12171500
• PubChem Substance ID: 329752209
• NACRES: MA.02

속성

• 양식: liquid
• Quality Level: 100
• 구성: fuchsin basic (certified stain; C.I. 42500), 3 % (w/w)
• 기술: microbe id | staining: suitable
• 불순물: phenol
• 색상: red to very dark red
• density: 0.99 g/mL at 20 °C
• ε (흡광계수): 77 at 546 nm in 50% ethanol
• 적합성: suitable for microscopy
• 응용 분야: diagnostic assay manufacturing; hematology; histology
• 저장 온도: room temp
• SMILES string: NC1=CC=C(C(C2=CC=C(N)C=C2)=C(C=C3)C=CC3=[NH2+])C=C1.[Cl-]
• InChI: 1S/C21H21N3/c1-13-11-16(5-9-19(13)23)21(15-3-7-18(22)8-4-15)17-6-10-20(24)14(2)12-17/h3-12,22H,23-24H2,1-2H3
• InChI key: IMOKRVRFBCWWGH-UHFFFAOYSA-N

설명

일반 설명

Carbol-Fuchsin is an acid-fast stain that consists of basic fuchsin and phenol. It is a dry staining dye for staining acid-fast bacteria such as Mycobacterium tuberculosis. Carbol-Fuchsin solution according to Kinyoun is a modified stain that does not require heat to infuse the carbol-fuchsin into the acid-fast cells during the staining process, and this process is also known as “cold staining”. It requires a higher concentration of the primary stain.

애플리케이션

• Kinyoun’s carbol-fuchsin is intended for use to stain paraffin sections of a formaldehyde-fixed specimen containing tubercle bacilli, and for the demonstration of tuberculosis bacteria (Mycobacterium tuberculosis).
• It is also applicable for other organisms that retain the dye after immersion in acidified 70% alcohol.
• It has been used to develop a staining technique for detecting microsporidial spores in fecal specimens.

원리

Acid-fast bacteria possess a waxy, lipid-rich cell wall that absorbs dyes very slowly. To enhance the absorption of carbol-fuchsin dye and the subsequent formation of the mycolate-fuchsin complex, the dye solution applied to the specimen is normally heated to the evaporation point. In Kinyoun’s modification of this method, the heating of the solution is avoided. Consequently, hazardous phenolic vapors are not released.

기타 정보

Stain used for the demonstration of tuberculosis bacteria

안전성 정보

• 픽토그램: GHS02,GHS08,GHS05,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H226,H302,H314,H341,H351,H412
• 예방조치 성명서: P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point (°F): 109.9 °F - closed cup
• Flash Point (°C): 43.3 °C - closed cup