114391
Bromophenol Blue
ACS reagent
동의어(들):
3′,3″,5′,5″-Tetrabromophenolsulfonphthalein, Bromphenol Blue Sultone Form
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모든 사진(3)
About This Item
실험식(Hill 표기법):
C19H10Br4O5S
CAS Number:
Molecular Weight:
669.96
Beilstein:
61698
EC Number:
MDL number:
UNSPSC 코드:
12171500
PubChem Substance ID:
NACRES:
NA.47
추천 제품
Grade
ACS reagent
Quality Level
양식
powder
기술
titration: suitable
visual transition interval
3.0-4.6, yellow to blue
mp
273 °C (lit.)
solubility
ethanol: 0.1%
응용 분야
diagnostic assay manufacturing
hematology
histology
저장 온도
room temp
SMILES string
Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4
InChI
1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H
InChI key
UDSAIICHUKSCKT-UHFFFAOYSA-N
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일반 설명
Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein.Bromophenol Blue is a pH indicator and a dye appearing as a strong blue color. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.
애플리케이션
Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose), and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. It has also been known for its use as a vital stain to probe the blood-brain barrier.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
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Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 28(1), 69-79 (2014-06-21)
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Dana Westphal et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(39), E4076-E4085 (2014-09-18)
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Helen Karlberg et al.
The Journal of general virology, 96(Pt 3), 538-546 (2014-12-08)
Pathogenesis of viral haemorrhagic fevers is associated with alteration of vascular barrier function and haemorrhage. To date, the specific mechanism behind this is unknown. Programmed cell death and regulation of apoptosis in response to viral infection is an important factor
Stephanie L Smith-Roe et al.
Oncotarget, 6(2), 732-745 (2014-12-30)
SWI/SNF complexes utilize BRG1 (also known as SMARCA4) or BRM (also known as SMARCA2) as alternative catalytic subunits with ATPase activity to remodel chromatin. These chromatin-remodeling complexes are required for mammalian development and are mutated in ~20% of all human
Siao-Syun Guan et al.
Oncotarget, 5(13), 4868-4880 (2014-06-21)
Colorectal cancer (CRC) is known as a common malignant neoplasm worldwide. The role of EGFR/HER2 in CRC is unclear. Afatinib is an irreversible EGFR/HER2 inhibitor. There were few studies of afatinib on CRC. Here, we investigated the protein levels/expressions of
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