POLYDIC-RO

Poly[d(I-C)]

lyophilized, pkg of 10 U (10108812001 [A₂₆₀ units]), pkg of 50 U (11219847001 [A₂₆₀ units])

• 동의어(들): Poly-deoxy-inosinic-deoxy-cytidylic acid
• UNSPSC 코드: 12352200

속성

• 설명: Poly-deoxy-inosinic-deoxy-cytidylic acid
• Quality Level: 100
• 양식: lyophilized
• 분자량: 28814 bp
• 포장: pkg of 10 U (10108812001 [A₂₆₀ units]); pkg of 50 U (11219847001 [A₂₆₀ units])
• 제조업체/상표: Roche
• 색상: white
• mp: 54 °C (NaCl, 0.1 M, pH 7.0)
• solubility: water: soluble
• 흡수비: A_{290/260 nm} 0.24-0.32; A_{280/260 nm} 0.61-0.67; A_{250/260 nm} 1.18-1.30
• 저장 온도: −20°C

설명

애플리케이션

Poly[d(I-C)] is used as a template for RNA polymerases, and as a DNA model for biophysical investigations.
It is also used as a primer template with Kornberg DNA polymerase for the determination of small amounts of dITP, dGTP, and dCTP. Poly(dI-dC) has been used in electrophoretic mobility shift assays (EMSA).

특징 및 장점

Contents
Lyophilizate, sodium salt, base ratio 1:1, alternating base sequence

품질

Typical analysis:
Absorbance determination A₂₅₀/A₂₆₀, A₂₈₀/A₂₆₀, A₂₉₀/A₂₆₀

서열

Chain Length approx. 20,000 bp

In an aqueous solution the DNA itself is a salt (the acid groups of the phosphate back bone have a negative charge and are saturated by positive sodium ions) i.e. there is no additional salt in solution. The structural formula which shows the negative 0-groups in the phosphate backbone is available. The same number of positive sodium ions must be dissociated in solution as counter ions.

As the sodium ions are part of the product and saturate the phosphate groups, the lyophilisate is salt-free.

단위 정의

1 A₂₆₀ unit corresponds to 50 μg ds DNA.

Unit Conversion: 1 A₂₆₀ unit corresponds to 50 μg ds DNA.

제조 메모

Activator: Polymines (spermine, spermidine)
Storage conditions (working solution): Solution is stable to at least 12 months if stored at 2 to 8 °C, contact with DNase or bacteria is to avoid.

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable