MABT356

Anti-Integrin alpha6 Antibody, clone 6H7.1

clone 6H7.1, from mouse

• 동의어(들): Integrin alpha-6, CD49 antigen-like family member F, VLA-6, CD49f
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 6H7.1, monoclonal
• 종 반응성: human
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_000201
• UniProt 수납 번호: P23229
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ITGA6(3655)

설명

일반 설명

Integrin alpha-6 (UniProt P23229; also known as CD49 antigen-like family member F, CD49f, Integrin alpha6B, VLA-6) is encoded by the ITGA6 (also known as ITGA6B) gene (Gene ID 3655) in human. The biological functions attributed to cell-extracellular matrix (ECM) interactions are mediated primarily by integrin family transmembrane receptors. A total of 18 alpha and 8 beta integrins have been identified in human. Each alpha integrin pairs up with one beta integrin non-covalently to form a functional heterodimeric dimer. At least 24 distinct integrin heterodimers are known with varying ligand specificities. For example, the alpha6-beta1 integrin is reported to mediate the spreading and laminin-binding of Schwann cells, while the alpha6-beta4 integrin is shown to play a role in the suppression of anoikis in human intestinal epithelial cells (IECs).

Signal (1-23), Integrin alpha-6 (24-1130). Processed into Integrin alpha-6 heavy chain (24 – 938), Integrin alpha-6 light chain (942-1130), Processed integrin alpha-6 (Alpha6p; 636 – 1130)

특이성

Expected to react with Integrin alpha-6 spliced isoforms 2 (Alpha-6X1A), 4 (Alpha-6X2A ), 6 (Alpha-6X1X2A), 7, and 9, as well as their respective proforms, cleaved light chains, and processed integrin alpha-6 forms (Alpha6p). Epitope is not present in isoform 1 (Alpha-6X1X2B), 3 (Alpha-6X1B), or 5 (Alpha-6X2B).

면역원

Epitope: Cytoplasmic region of isoforms 2/4/6/7/9

KLH-conjugated linear peptide corresponding to the cytoplasmic region of human Integrin alpha6 isoforms 2/4/6/7/9.

애플리케이션

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Integrin alpha6 in human skin tissue.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

This Anti-Integrin alpha6 Antibody, clone 6H7.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Integrin alpha6.

품질

Evaluated by Western Blotting in SW480 human colon cancer cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Integrin alpha6 in 10 µg of SW480 human colon cancer cell lysate.

표적 설명

~140 kDa observed. Due to glycosylation, the target band(s) appears larger than the calculated 106.6 to 124.0 kDa based on the proform sequences of isoforms 2/4/6/7/9. Uncharacterized band(s) may appear in some lysates.

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable