MABS1212

Anti-Geminin Antibody, clone 4B10.1

clone 4B10.1, from mouse

• 동의어(들): Geminin
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 4B10.1, monoclonal
• 종 반응성: human
• 기술: immunocytochemistry: suitable; western blot: suitable
• 동형: IgG2aκ
• NCBI 수납 번호: NP_001238918
• UniProt 수납 번호: O75496
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... GMNN(51053)

설명

일반 설명

Geminin (UniProt O75496) is encoded by the GMNN gene (Gene ID 51053) in human. Geminin is a negative regulator of DNA-replication and the founding member of the Geminin family, whose members also include Idas (multicilin; McIdas) and GemC1 (Geminin coiled-coil domain-containing protein 1). Geminin binds Cdt1 and inhibits the mini-chromosome maintenance complex (MCM) loading onto chromatin and pre-replication complex (preRC) formation. Geminin is a 209-amino acid protein that can form homodimer or heterodimers with either Idas or GemC1 via the middle coiled coil structure (a.a. 94-144 of Geminin) in a head-to-tail fashion. The same region in Geminin (a.a. 82-161) is also essential for Cdt1 interaction. A homeodomain-binding domain is located at the C-terminal region of Geminin (a.a. 170-190). GemC1 is also implicated in DNA replication but at a later stage than Geminin. All three Geminin family members play important roles in cell proliferation and differentiation.

특이성

Clone 4B10.1 detects human geminin by targeting an internal epitope.

면역원

GST-tagged recombinant human geminin internal fragment.

애플리케이션

Detect Geminin using this mouse monoclonal Anti-Geminin, clone 4B10.1 Antibody, Cat. No. MABS1212, validated for use in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected nuclear geminin immunoreactivity in HUVECs, A431, and HeLa cells.

Research Category
Signaling

품질

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected geminin in 10 µg of HEK293 cell lysate.

표적 설명

~31 kDa observed. 23.57 kDa calculated. The larger-than-predicted band size is consistent with that reported in the literature (McGarry, T.J., and Kirschner, M.W. (1998). Cell. 93(6):1043-1053 ). Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified

Protein G purified.

Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable