추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
ATAP2, monoclonal
종 반응성
human, mouse
기술
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable
동형
IgG1κ
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... F2R(2149)
일반 설명
The protein named Proteinase-activated receptor 1 (PAR-1/PAR1) or Coagulation factor II receptor, or Thrombin receptor and encoded by the human gene F2R/CR2R/PAR1/TR is a high affinity receptor for activated thrombin and part of the innate immune system response driven by proteinase-activated receptors (PARs). PARs are G protein-coupled receptors that transmit cellular responses to extracellular proteases and have important functions in vascular physiology, development, inflammation, and cancer progression. PAR1 is important for platelet activation and vascular development. PAR1 expression is limited to platelets and vascular endothelial cells and is localized to the cell membrane. Interestingly too, even the signal peptide of PAR1 (which guides the protein to the membrane) plays a role in cell signaling as if forms the intracellular angiogenesis inhibitor peptide Parstatin.
면역원
Linear peptide corresponding to human Par-1.
애플리케이션
Flow Cytometry Analysis: 4 µg from a representative lot detected Par-1 in 1X10E6 human thrombocytes (platelets).
Flow Cytometry Analysis: A representative lot from an independent laboratory detected Par-1 in CHRF-288 and HEL cells (Hoxie, J. A., et al. (1993). J Biol Chem. 268(18):13756-13763.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Par-1 in human platelet membrane cell lysate (Brass, L. F., et al. (1992). J Biol Chem. 267(20):13795-13798.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Par-1 in CHRF-288 and HEL cells (Hoxie, J. A., et al. (1993). J Biol Chem. 268(18):13756-13763.).
Immunofluorescence Analysis: A representative lot from an independent laboratory detected Par-1 in CHRF-288 cells (Brass, L. F., et al. (1994). J Biol Chem. 269(4):2943-2952.).
Flow Cytometry Analysis: A representative lot from an independent laboratory detected Par-1 in CHRF-288 and HEL cells (Hoxie, J. A., et al. (1993). J Biol Chem. 268(18):13756-13763.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Par-1 in human platelet membrane cell lysate (Brass, L. F., et al. (1992). J Biol Chem. 267(20):13795-13798.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Par-1 in CHRF-288 and HEL cells (Hoxie, J. A., et al. (1993). J Biol Chem. 268(18):13756-13763.).
Immunofluorescence Analysis: A representative lot from an independent laboratory detected Par-1 in CHRF-288 cells (Brass, L. F., et al. (1994). J Biol Chem. 269(4):2943-2952.).
This Anti-PAR-1 antibody, clone ATAP2 is validated for use in western blotting, flow cytometry, ICC & immunofluorescence for the detection of PAR-1.
품질
Evaluated by Western Blotting in mouse lymph node tissue.
Western Blotting Analysis: 1 µg/mL of this antibody detected Par-1 in 10 µg of mouse lymph node tissue.
Western Blotting Analysis: 1 µg/mL of this antibody detected Par-1 in 10 µg of mouse lymph node tissue.
표적 설명
~50 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
물리적 형태
Format: Purified
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Internalization and recycling of activated thrombin receptors.
Hoxie, J A, et al.
The Journal of Biological Chemistry, 268, 13756-13763 (1993)
L F Brass et al.
The Journal of biological chemistry, 269(4), 2943-2952 (1994-01-28)
According to current models, human thrombin receptors are activated when thrombin cleaves the receptor's N terminus, exposing the tethered ligand domain, SFLLRN. In the megakaryoblastic CHRF-288 cell line, thrombin receptor activation is followed by the rapid internalization of > 90%
L F Brass et al.
The Journal of biological chemistry, 267(20), 13795-13798 (1992-07-25)
Based upon its recently cloned nucleotide sequence, the human platelet thrombin receptor is thought to be formed by a single polypeptide chain with seven transmembrane domains and an extracellular N terminus that can be cleaved by thrombin. As yet, however
Xiaofeng Cai et al.
Journal of thrombosis and haemostasis : JTH, 17(5), 803-817 (2019-03-14)
Essentials APC elicits cytoprotective responses in endothelial cells via EPCR-dependent cleavage of PAR1. APC inhibits LPS-mediated translocation and extracellular secretion of HMGB1 in endothelial cells. Signaling activity of APC inhibits LPS-mediated acetylation of HMGB1 by epigenetic mechanisms. APC inhibits LPS-mediated
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.