MABC1022

Anti-BCL2L12 Antibody, clone 11B5.1

clone 11B5.1, from mouse

• 동의어(들): Bcl-2-like protein 12
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 11B5.1, monoclonal
• 종 반응성: human
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_619580
• UniProt 수납 번호: Q9HB09
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... BCL2L12(83596)

설명

일반 설명

Bcl-2-like protein 12 (UniProt Q9HB09; also known as Bcl-2-related proline-rich protein, Bcl2-L-12) is encoded by the BCL2L12 (also known as BPR) gene (Gene ID 83596) in human. Bcl-2 family proteins paly either a pro- or anti-apoptotic role in cell death signaling. BCL-2 family members are related by regions of sequence and structural homology. The pro-survival members contain four BCL-2 homology regions (BH1 BH4), whereas the BH3-only members contain only the BH3 amphipathic helix that mediates their interaction with the groove of multi-domain BCL-2 family members. Bcl2-L-12 is an anti-apoptotic protein that shares a lower amino acid similarity with the other Bcl2 family members. It contains a BH2 (a.a. 311-322) domain and a BH3-like domain (a.a. 192-220) that mediate its interaction with Bcl2 and Bcl-xL. Exogenous expression of Bcl2-L-12 with Leu213 or Leu217 mutation in U87MG cell line is reported to result in reactivation of PARP cleavage, caspase-3 and cytochrome c release compared to cells expressing wild-type Bcl2-L-12.

특이성

Clone 11B5.1 targets an internal epitope present in spliced isoforms 1&3, but absent in isoform 2, of human BCL2L12 reported by UniProt (Q9HB09).

면역원

GST-tagged recombinant human BCL2L12 internal fragment.

애플리케이션

Detect Bcl2L12 using this mouse monoclonal Anti-BCL2L12, clone 11B5.1 Antibody, Cat. No. MABC1022, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected BCL2L12 in human pancreas, skeletal muscle, and tonsil tissue sections.

Research Category
Apoptosis & Cancer

품질

Evaluated by Western Blotting in PC3 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected BCL2L12 in 10 µg of PC3 human prostate cancer cell lysate.

표적 설명

~37 kDa observed. 36.82/36.67 kDa (isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified

Protein G purified.

Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable