추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
6C4, monoclonal
종 반응성
monkey
종 반응성(상동성에 의해 예측)
mouse, rat, canine
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable
동형
IgG2a
NCBI 수납 번호
UniProt 수납 번호
타겟 번역 후 변형
unmodified
유전자 정보
dog ... Gria2(482667)
mouse ... Gria2(14800)
rat ... Gria2(29627)
rhesus monkey ... Gria2(574344)
일반 설명
Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. Although subunit composition of AMPA receptors varies, they must contain at least one edited GluR2 subunit to be calcium impermeable. The critical residue controlling calcium permeability is in the pore loop region. In GluR1, GluR3, and GluR4, this positionis occupied by a Gln residue. In GluR2, it is occupied by an Arg residue. It has been shown experimentally that the presence of Arg in this position blocks CA2+ ion permeability, while a Gln does not. Relative calcium permeability in AMPA receptor channels may be significant in pathological neurotoxic damage and long term changes in nervous system responses.
특이성
Recognizes the large N-terminal extracellular domain of Glutamate Receptor 2 (GluR2). No cross-reactivity observed with other AMPA/Kainate GluR subunits. On western blots of brain extracts from rat, macaque monkey, and dog, MAB397 recognizes a band at approximately 102 kDa corresponding to full length GluR2. Other proteins noted by Western blot at 66 kDa or lower molecular weight appear to be breakdown products of GluR2 (Vissavajjhala, 1996). By immunohistochemistry GluR2 is widely distributed at both the cellular and synaptic levels. MAB397 recognizes GluR2 present in a vast majority of, but not all, GABAergic interneurons (Vissavajjhala, 1996).
애플리케이션
Anti-Glutamate Receptor 2 Antibody, extracellular, clone 6C4 detects level of Glutamate Receptor 2 & has been published & validated for use in ELISA, IC, IH, IP, RIA & WB with more than 50 product citations.
Immunocytochemistry:
on 4% paraformaldehyde fixed cells was used in a previous lot:2-3 µg/mL (Vissavajjhala, 1996; Osten, 1998; Passafaro, 2001).
ELISA/RIA:
A previous lot of this antibody was used in ELISA/RIA.(Vissavajjhala, 1996).
Immunohistochemistry on 50 µm, 4% paraformaldehyde fixed brain sections: 1:500-1:800 (Vissavajjhala, 1996; Yung, 1998; Kumar; 2002).
Immunoprecipitation: 2-4 µg/mL (Osten, 1998).
Western blot: 1-2 µg/mL (Vissavajjhala, 1996; Osten, 1998); membrane preparations are suggested for enhanced signals.
Optimal working dilutions and protocols must be determined by end user.
on 4% paraformaldehyde fixed cells was used in a previous lot:2-3 µg/mL (Vissavajjhala, 1996; Osten, 1998; Passafaro, 2001).
ELISA/RIA:
A previous lot of this antibody was used in ELISA/RIA.(Vissavajjhala, 1996).
Immunohistochemistry on 50 µm, 4% paraformaldehyde fixed brain sections: 1:500-1:800 (Vissavajjhala, 1996; Yung, 1998; Kumar; 2002).
Immunoprecipitation: 2-4 µg/mL (Osten, 1998).
Western blot: 1-2 µg/mL (Vissavajjhala, 1996; Osten, 1998); membrane preparations are suggested for enhanced signals.
Optimal working dilutions and protocols must be determined by end user.
품질
Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected glutamate receptor 2 on 10 μg of mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected glutamate receptor 2 on 10 μg of mouse brain lysates.
표적 설명
102 kDa
물리적 형태
Format: Purified
Purified immunoglobulin from culture supernatant
Purified mouse immunoglobin IgG2a liquid in buffer containing PBS, no preservative.
저장 및 안정성
Stable for 6 months at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage the IgG2a and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage the IgG2a and affect product performance.
분석 메모
Control
Positive Control: Cerebral cortex and pyramidal neurons, mouse brain lysate.
Positive Control: Cerebral cortex and pyramidal neurons, mouse brain lysate.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
A developmental switch of AMPA receptor subunits in neocortical pyramidal neurons.
Sanjay S Kumar, Alberto Bacci, Viktor Kharazia, John R Huguenard
The Journal of Neuroscience null
Reversible paraneoplastic limbic encephalitis associated with antibodies to the AMPA receptor.
Bataller L, Galiano R, Garcia-Escrig M, Martinez B, Sevilla T, Blasco R, Vilchez JJ, Dalmau J
Neurology null
Contribution of the global subunit structure and stargazin on the maturation of AMPA receptors.
NF Shanks, T Maruo, AN Farina, MH Ellisman, T Nakagawa
The Journal of Neuroscience null
Tyrosine phosphatases regulate AMPA receptor trafficking during metabotropic glutamate receptor-mediated long-term depression.
Moult, PR; Gladding, CM; Sanderson, TM; Fitzjohn, SM; Bashir, ZI; Molnar, E; Collingridge, GL
The Journal of Neuroscience null
Tyrosine phosphorylation and regulation of the AMPA receptor by SRC family tyrosine kinases.
Hayashi, T; Huganir, RL
The Journal of Neuroscience null
문서
The term neurodegeneration characterizes a chronic loss of neuronal structure and function leading to progressive mental impairments.
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