GF023

Tumor Necrosis Factor-α Protein, Recombinant human

Tumor Necrosis Factor-alpha (TNF-alpha) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells & certain other target cells.

• 동의어(들): TNF-alpha
• UNSPSC 코드: 12352202
• eCl@ss: 32160405
• NACRES: NA.75

속성

• 생물학적 소스: human
• Quality Level: 100
• 분석: >97% (SDS-PAGE)
• 특이 활성도: ≥2 x 107 U/mg
• 제조업체/상표: Chemicon^®
• 기술: cell culture | mammalian: suitable
• 입력: sample type hematopoietic stem cell(s)
• NCBI 수납 번호: NM_000594.2
• UniProt 수납 번호: P01375
• 배송 상태: dry ice
• 유전자 정보: human ... TNF(7124)

설명

일반 설명

Product Source: E.coli

Tumor Necrosis Factor-alpha (TNF-alpha) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. The mature form of human TNF-alpha has 157 amino acid residues. GF023 is a recombinant polypeptide containing the mature form of human TNF-a with a polyhistidine tag at its amino terminal.

애플리케이션

For most in vitro applications, TNF-alpha exerts its biological activity in the concentration range of 0.05 to 20.0 ng/mL. Responding cells are (partial list): Tumor cells, certain other cells.

물리적 형태

Lyophilized from 3mM Tris, pH 8.0.

저장 및 안정성

The lyophilized powder, though stable at room temperature, is best stored desiccated at -20°C. Reconstitute with sterile distilled water to a concentration of 0.1-1 mg/mL. Note: Allow the reconstituted vial to sit at room temperature for 2 hours before use. This solution can be diluted into other buffered solutions and stored at 4°C for one week or -20°C for future use. Reconstituted TNF-alpha should be stored in undiluted aliquots at -20°C for up to six months.

분석 메모

Specific Activity: Chemicon′s human TNF-alpha, is fully biologically active when compared to standards.The ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin-D is ≤ 0.05 ng/mL

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3