추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
serum
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
mouse, human
제조업체/상표
Chemicon®
기술
flow cytometry: suitable
western blot: suitable
입력
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... NANOG(79923)
mouse ... Nanog(71950)
일반 설명
NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency. In other words, these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).
특이성
Recognizes Nanog, human.
면역원
Synthetic peptide from human Nanog.
애플리케이션
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
This Anti-Nanog Antibody is validated for use in FC, WB for the detection of Nanog.
Western blotting: 1:1,000-1:5,000. The antibody recognizes a protein of ~35 kDa on MBL-5 cell lysate. Additional bands of ~50 and ~60 kDa may be seen depending on sample and antibody dilution used.
Flow Cytometry: Cormier et al., 2006. Tissue Engineering 12:3233-3245.
Optimal working dilutions must be determined by the end user.
Flow Cytometry: Cormier et al., 2006. Tissue Engineering 12:3233-3245.
Optimal working dilutions must be determined by the end user.
표적 설명
39 kDa (MW 34 kDa)
물리적 형태
Rabbit Serum. Liquid.
Unpurified
저장 및 안정성
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
분석 메모
Control
Whole cell lysate from mouse embryonic stem cells
Whole cell lysate from mouse embryonic stem cells
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
A genome-wide RNAi screen identifies a new transcriptional module required for self-renewal.
Hu, G; Kim, J; Xu, Q; Leng, Y; Orkin, SH; Elledge, SJ
Genes & Development null
Guixiang Tan et al.
PloS one, 9(4), e92304-e92304 (2014-04-20)
Activation of signal transducer and activator of transcription 3 (Stat3) by leukemia inhibitory factor (LIF) is required for maintaining self-renewal and pluripotency of mouse embryonic stem cells (mESCs). Here, we have confirmed transcription factor Forkhead Box m1 (Foxm1) as a
Culture conditions and enzymatic passaging of bovine ESC-like cells.
Guochun Gong,Marsha L Roach,Le Jiang,Xiangzhong Yang,Xiuchun Cindy Tian
Cellular Reprogramming null
[Molecular genetic characterization of the regulatory region of the Xist gene in the common vole Microtus rossiaemeridionalis].
K E Orishchenko,E A Elisafenko,A E Kel',S M Zakian
Genetika null
Matthew J Simmons et al.
Breast cancer research : BCR, 14(5), R126-R126 (2012-09-21)
NOTCH activation has been recently implicated in human breast cancers, associated with a poor prognosis, and tumor-initiating cells are hypothesized to mediate resistance to treatment and disease relapse. To address the role of NOTCH1 in mammary gland development, transformation, and
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