70850

FastPlax Titer Kit - Novagen

Rapid, reliable titering of baculovirus stocks

• UNSPSC 코드: 12352200
• NACRES: NA.54

속성

• Quality Level: 100
• 제조업체/상표: Novagen^®
• 저장 조건: OK to freeze

설명

일반 설명

The FastPlax Titer Kit is designed for determination of baculovirus titers in just 24 to 48 hours instead of three to four days as in other methods. Classical determination of baculovirus titer has relied on the appearance of plaques, but the FastPlax Kit takes advantage of the appearance of detectable levels of AcNPV gp64 glycoprotein on the cell surface as early as 8 to 24 hours postinfection. Detection is based on a high affinity monoclonal antibody against gp64, which is added directly to fixed cells. Antibody binding is detected with Goat Anti-Mouse IgG β-Galactosidase Conjugate followed by enhanced color development with X-Gal/NBT substrates. Plaques are clearly distinguished by their dark blue color. The kit contains sufficient reagents to perform five 6-well plate assays.

포장

Plastic ampoule

성분

• 2 × 50 ml- 10X TBST (100 mM Tris-HCl, pH 8.0, 1.5 M NaCl, 1% Tween^® 20)
• 6 ml- 10% Gelatin (contains 0.02% azide)
• 10 µl- FastPlax Antibody
• 300 µl- Goat-Anti-Mouse β-Galactosidase Conjugate
• 300 µl- NBT (83 mg/ml in 70% dimethylformamide)
• 300 µl- X-gal (50 mg/ml in dimethylformamide)

경고

Toxicity: Multiple Toxicity Values, refer to MSDS

저장 및 안정성

Multiple storage requirements

법적 정보

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

안전성 정보

• 픽토그램: GHS02,GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H226,H312 + H332,H319,H360D
• 예방조치 성명서: P210 - P280 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B
• Storage Class Code: 3 - Flammable liquids