324708

Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi

• 동의어(들): Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi
• CAS Number: 9001-92-7
• EC 번호: 3.4.24.33 (BRENDA, IUBMB)
• MDL number: MFCD00004464
• UNSPSC 코드: 12352202
• NACRES: NA.54

속성

• Grade: Proteomics Grade
• Quality Level: 100
• 분석: ≥90% (SDS-PAGE)
• 양식: lyophilized solid
• 특이 활성도: ≥20,000 units/mg protein
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 색상: white
• solubility: water: soluble
• 배송 상태: ambient
• 저장 온도: 2-8°C

설명

일반 설명

Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

경고

Toxicity: Harmful (C)

단위 정의

One unit is defined as the amount of enzyme that will release a sufficient amount of azo dye from AZOCOLL Substrate (Cat. No. 194932) to produce an increase in absorbance at 520 nm of 0.001 per min at 37°C, pH 7.5.

물리적 형태

Lyophilized from 10 mM Tris-HCl, pH 7.5.

재구성

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.

기타 정보

Drapeau, G.R. 1980. J. Biol. Chem.255, 839.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H334,H335
• 예방조치 성명서: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1