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Merck
모든 사진(1)

주요 문서

324390

Sigma-Aldrich

Anti-Dopamine D₁ Receptor Rabbit pAb

lyophilized, Calbiochem®

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

항체 형태

serum

항체 생산 유형

primary antibodies

클론

polyclonal

양식

lyophilized

미포함

preservative

종 반응성

human, mouse, rat

제조업체/상표

Calbiochem®

저장 조건

OK to freeze

동형

IgG

배송 상태

ambient

저장 온도

2-8°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... DRD1(1812)
rat ... Drd1(24316)

일반 설명

Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~50 kDa dopamine D1 receptor protein.
Recognizes the dopamine D1 receptor in rat brain. Does not cross-react with other dopamine receptors.
This Anti-Dopamine D₁ Receptor Antibody is validated for use in ELISA, WB, Frozen Sections, Free-Floating Sections, Paraffin Sections for the detection of Dopamine D₁ Receptor.

면역원

Human
a synthetic peptide (MDGTGLVVERDFSC) corresponding to amino acids 9-21 of human D₁dopamine receptor

애플리케이션

ELISA (see comments)

Immunoblotting (1:1000)

Frozen Sections (1:5000)

Free-Floating Sections (1:5000)

Paraffin Sections (not recommended)

경고

Toxicity: Standard Handling (A)

물리적 형태

Undiluted serum.

재구성

Reconstitute the antibody with 100 µl PBS containing 1% BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).

분석 메모

Positive Control
Rat brain

기타 정보

Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.
This antibody has also been reported to work in ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat′s milk. For frozen sections a 1:5000 dilution of the reconstituted serum demonstrates specific staining of rat brain sections including the medial septum, nucleus accumbens, dentate gyrus, globus pallidus, medial forebrain bundle, cortex regions 1-3, substantia nigra reticulata, and the ventral tegmental area. Strong nuclear staining of oligodendrocytes was also observed. Variables associated with assay conditions will dictate the proper working dilution.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Jacqueline S Coley et al.
PloS one, 10(2), e0117450-e0117450 (2015-02-04)
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Tina M Calderon et al.
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, 12(2), 353-370 (2017-01-31)
In human immunodeficiency virus-1 (HIV) infected individuals, substance abuse may accelerate the development and/or increase the severity of HIV associated neurocognitive disorders (HAND). It is proposed that CD14+CD16+ monocytes mediate HIV entry into the central nervous system (CNS) and that
Florian Giesert et al.
PloS one, 8(5), e63778-e63778 (2013-05-16)
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