추천 제품
생물학적 소스
rabbit
Quality Level
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
human
기술
ELISA: suitable
immunocytochemistry: suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... DNMT3A(1788)
일반 설명
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. DNMT3 is a developmentally regulated DNA methyltransferase. DNMT3A2 is a second isoform of DNMT3 that is expressed from an alternative promoter. DNMT3A2 localizes to euchromatin and is thought to function in de novo methylation, rather than maintenance methylation. Binding of DNMT3L (DNMT3-like protein) to DNMT3A2 promotes a dramatic reorganization of DNMT3A2 subunits and leads to the formation of specific complexes with enhanced DNA methyltransferase activity. DNMT3A2 targets endogenous DNMT3L to embryonic stem cell chromatin and induces regional DNA methylation.
특이성
Other homologies: Predicted to react with Mouse and Rat based on 100% sequence homology
The antibody recognizes isoform 2 of human DNMT3A. Additional lower molecular weight bands are detected in some samples.
면역원
Epitope: C-terminus
The immunogen was a KLH-conjugated synthetic peptide corresponding to the C-terminal region of human DNMT3A2.
애플리케이션
ELISA:
This antibody was shown by an outside laboratory to be suitable for ELISA.
Immunocytochemistry:
This antibody detects DMNT3A2 in the nuclei of NIH/3T3, HeLa, and A431 cells.
This antibody was shown by an outside laboratory to be suitable for ELISA.
Immunocytochemistry:
This antibody detects DMNT3A2 in the nuclei of NIH/3T3, HeLa, and A431 cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Use Anti-DNMT3A2 Antibody (Rabbit Polyclonal Antibody) validated in ICC, ELISA, WB to detect DNMT3A2 Antibody also known as DNA (cytosine-5)-methyltransferase 3 alpha.
품질
Western Blot Analysis: 1:1,000 dilution of this antibody detected DNMT3A2 on 10 µg of L6 lysate.
표적 설명
~80 kDa
물리적 형태
Format: Purified
Protein A chromatography
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
분석 메모
Control
L6, NIH/3T3, HeLa, or A431 cells.
L6, NIH/3T3, HeLa, or A431 cells.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Michael S Kareta et al.
The Journal of biological chemistry, 281(36), 25893-25902 (2006-07-11)
The DNMT3-like protein, DNMT3L, is required for germ line DNA methylation, although it is inactive as a DNA methyltransferase per se. Previous studies have shown that DNMT3L physically associates with the active de novo DNA methyltransferases, DNMT3A and DNMT3B, and
Keisuke Nimura et al.
Genes to cells : devoted to molecular & cellular mechanisms, 11(10), 1225-1237 (2006-09-27)
DNA methylation is involved in fundamental cellular processes such as silencing of genes and transposable elements, but the underlying mechanism of regulation of DNA methylation is largely unknown. DNA methyltransferase 3-like protein (Dnmt3L), a member of the Dnmt3 family of
Taiping Chen et al.
The Journal of biological chemistry, 277(41), 38746-38754 (2002-07-26)
Previous studies have shown that the Dnmt3b gene encodes multiple variants via alternative splicing. However, only one form of Dnmt3a has been identified to date. We report here the discovery of a small form of Dnmt3a, denoted Dnmt3a2, from both
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