04-841

Anti-ROKα/ROCK-II Antibody, clone A9W4, rabbit monoclonal

culture supernatant, clone A9W4, from rabbit

• 동의어(들): RhoA-binding kinase 2, Rho-associated, coiled-coil containing protein kinase 2, Rho-associated, coiled-coil-containing protein kinase II, p150 ROK-alpha, p164 ROCK-2, ROCK-II, ROKalpha, Rock2
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: culture supernatant
• 항체 생산 유형: primary antibodies
• 클론: A9W4, monoclonal
• 종 반응성: rat, human, mouse, bovine
• 기술: immunoprecipitation (IP): suitable; western blot: suitable
• NCBI 수납 번호: NM_013022
• UniProt 수납 번호: Q62868
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: rat ... Rock2(25537)

설명

일반 설명

Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKα/ROCK-II and ROKβ/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin.

특이성

Predicted to cross-react with mouse and bovine based on sequence homology.

Recognizes ROKα/ROCK-II, Mr 150 kDa. Does not recognize ROKβ/ROCK-I. A non-specific band may also be detected, Mr 80 kDa.

면역원

6His-tagged fusion protein corresponding to residues 2-543 of rat ROKα/ROCK-II.

애플리케이션

Detect ROKα/ROCK-II using this Anti-ROKα/ROCK-II Antibody, clone A9W4 validated for use in IP & WB.

Immunoprecipitation:
4 μL of a previous lot immunoprecipitated ROKα/ROCK-II from 500 μg of PC-12 lysate.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

품질

Routinely evaluated by Western BLot on RIPA lysates from PC-12 cells.

Western Blot Analysis: A 1:500-1:5000 dilution of this antibody detected ROKα/ROCK-II in RIPA lysates from PC-12 cells.

표적 설명

150 kDa

결합

Replaces: 05-841

물리적 형태

Cultured supernatant containing 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

분석 메모

Control
RIPA lysates from PC-12 cells

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable