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K3643

Sigma-Aldrich

PKAcγ, active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

KAPG, PKAr, cAPKr

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

recombinant

expressed in baculovirus infected Sf9 cells

Quality Level

product line

PRECISIO® Kinase

Assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

128-174 nmol/min·mg

mol wt

~65 kDa

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PRKACG(5568)

Biochem/physiol Actions

PKAcγ (protein kinase cAMP-activated catalytic subunit γ) is a catalytic isoform of cyclic adenosine monophosphate-dependent protein kinase. It is an intronless gene and has a testis-specific expression. It is involved in platelet biogenesis. Mutation in PKAcγ causes macrothrombocytopenia, an inherited thrombocytopenias.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Vladimir T Manchev et al.
Blood, 124(16), 2554-2563 (2014-07-26)
Macrothrombocytopenias are the most important subgroup of inherited thrombocytopenias. This subgroup is particularly heterogeneous because the affected genes are involved in various functions such as cell signaling, cytoskeleton organization, and gene expression. Herein we describe the clinical and hematological features
S J Beebe et al.
Molecular endocrinology (Baltimore, Md.), 4(3), 465-475 (1990-03-01)
Two different mammalian genes for the catalytic subunit (C) of cAMP-dependent protein kinase have previously been characterized (C alpha, C beta). In the present study, we report the molecular cloning of a third isoform of C, from a human testis
N Reinton et al.
Genomics, 49(2), 290-297 (1998-05-23)
Three different catalytic isoforms of cAMP-dependent protein kinase have been identified (C alpha, C beta, and C gamma). We report the cloning and characterization of the human and rhesus monkey genes encoding the testis-specific C gamma subunit. The human C

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