S5171

Human Fibronectin

formulated with recombinant human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma, liquid, 2 mg/mL, suitable for cell culture

• 別名: sFN
• MDL番号: MFCD00284706
• UNSPSCコード: 12352202
• NACRES: NA.75

特性

• Product Name: スーパーフィブロネクチン ヒト血漿由来, solution, BioReagent, suitable for cell culture
• 由来生物: human
• 品質水準: 200
• リコンビナント: expressed in E. coli
• 製品種目: BioReagent
• フォーム: solution
• 分子量: ~235-270 kDa
• 包装: pkg of 5 mg
• テクニック: cell culture | mammalian: suitable
• 表面被覆率: 1 μg/cm²
• UniProtアクセッション番号: P02751
• 輸送温度: ambient
• 保管温度: 2-8°C
• 遺伝子情報: human ... FN1(2335)

詳細

アプリケーション

Superfibronectin from human plasma dramatically enhances the adhesive properties of fibronectin and suppresses cell migration. Improved results have been shown CHO cells, IMR-90 fibroblasts, T47D breast carcinoma cells, and UCLA-P lung carcinoma cells. Additional applications include epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at 1 μg/cm2.

構成

原材料はHBsAgおよびHIV抗体に対して試験されています。

注意

Superfibronectin should be stored at 2-8°C and diluted immediately prior to use. Do not freeze this product.

調製ノート

As supplied, this product is a 2 mg protein/mL solution in 0.05 M Tris buffered saline. Before coating a 96 well plate, it is recommended to dilute t he product with sterile PBS to a concentration of 5 μg/mL and add 100 μl/well. These plates should then be incubated for 2 hours at 37°C and washed twice with 100 μl of PBS. The source material has been tested for HBsAg and HIV antibodies.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable