NDEGLY

Native Protein Deglycosylation Kit

• 別名: Protein Deglycosylation Kit
• UNSPSCコード: 12352202
• NACRES: NA.32

特性

• 結合体: (N-linked)
• 品質水準: 200
• 輸送温度: wet ice
• 保管温度: 2-8°C

詳細

詳細

All complex oligosaccharides can be reduced to the trimannosylchitobiose core by treatment of the glycoproteins with neuraminidase, β-galactosidase, and N-acetylglucosaminidase. Fucosidases may be required in some situations. The remaining trimannosylchitobiose core structures can be removed with endoglycosidase F3. Biantennary and triantennary structures can be immediately removed by endoglycosidases F2 and F3, respectively. Oligomannose and hybrid structures can be removed by Endoglycosidase F1.
For more information on each type of endoglycosidase, please refer to the Bulletin.

The Native Protein Deglycosylation is designed for the deglycoslylation of N-linked oligosaccharides from PNGase F-resistant native proteins. Endoglycosidases F1, F2, and F3 are less sensitive to protein conformation than PNGase F and are more suitable for removal of all classes of N-linked oligosaccharides without protein denaturation.

アプリケーション

Native Protein Deglycosylation Kit has been for N-deglycosylation of various enzymes such as laccase, tomato nuclease TBN1 and endo-β-1,3-glucanase.

保管および安定性

The NDEGLY Kit ships on wet ice and storage at 2–8 °C is recommended. This kit may be used for at least 1 year when stored as indicated. Several days exposure to ambient temperatures will not reduce the activity of the enzymes in this kit.

キットの構成要素のみ

• Endoglycosidase F1 .3 U
• Endoglycosidase F2 .1 U
• Endoglycosidase F3 .1 U
• Endoglycosidase F1 reaction buffer 200 μL
• Endoglycosidase F2 & 3 reaction buffer 200 μL

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable