EMS0001

PNGase Fast

recombinant, expressed in E. coli

• 別名: N-Glycosidase F, PNGase F, Peptide N-glycosidase
• UNSPSCコード: 41131616

特性

• リコンビナント: expressed in E. coli
• 品質水準: 200
• 結合体: (N-linked)
• グレード: Proteomics Grade
• フォーム: ready-to-use solution
• 輸送温度: wet ice
• 保管温度: 2-8°C

詳細

詳細

Peptide-N-glycosidase F (PNGase F) belongs to an enzyme family, that are mainly used for the deglycosylation of N-linked glycans.

アプリケーション

PNGase Fast may be used to immobilize in order to perform deglycosylation. It may also be used to immobilize onto methacrylate based monolithic support to release the N-linked carbohydrate moieties from glycoproteins.

生物化学的／生理学的作用

Peptide-N-glycosidase F (PNGase F) cleaves asparagine-linked high mannose, hybrid and complex oligosaccharides from glycoproteins. It can also deaminate the asparagine to aspartic acid. PNGase Fast enables complete and rapid deglycosylation of antibodies and immunoglobulin fusion proteins, as well as other glycoproteins, to be prepared for downstream chromatography or mass spectrometry analysis. PNGase Fast creates an optimized workflow, reducing processing time without compromising sensitivity or reproducibility.

安全性情報

• 保管分類コード: 10 - Combustible liquids