DUO82064

Duolink^® In Situ Microplate Nuclear Stain, Anti-Fade

• 別名: in situ Proximity Ligation Assay reagent, Protein Protein Interaction Assay reagent
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 製品種目: Duolink^®
• 品質水準: 200
• テクニック: proximity ligation assay: suitable
• 蛍光検出: λ_ex 360 nm; λ_em 460 nm
• 適合性: suitable for fluorescence-detection automated sequencing; suitable for microtiter plates
• 輸送温度: dry ice
• 保管温度: −20°C

詳細

アプリケーション

Duolink^®proximity ligation assay(PLA^®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Use the Multiwell Plates modifications to the Duolink^® In Situ Fluorescence Protocol to run an experiment with this product. A set of short instructions can also be used.

Visit our Duolink^® PLA Resource Center for information on how to run a Duolink^® experiment, applications, troubleshooting, and more.

To perform a complete Duolink^® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink^® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.

Specificity
Duolink^® In Situ Microplate Nuclear Stain and Anti-Fade are intended to be used after staining cells with Duolink^® In Situ in microtiter plates. See the datasheet for more information.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink^® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

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特徴および利点

• No overexpression or genetic manipulation required
• High specificity (fewer false positives)
• Single molecule sensitivity due to rolling circle amplification
• Relative quantification possible
• No special equipment needed
• Quicker and simpler than FRET
• Increased accuracy compared to co-IP
• Publication-ready results

法的情報

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany

PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H317,H412
• 注意書き: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• 危険有害性の分類: Aquatic Chronic 3 - Skin Sens. 1
• 保管分類コード: 12 - Non Combustible Liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable