A2237

アルカリホスファターゼ エビ由来

≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host

• 別名: オルトリン酸-モノエステルホスホヒドロラーゼ(アルカリ性至適)
• CAS Number: 9001-78-9
• EC Number: 3.1.3.1 (BRENDA, IUBMB)
• MDL番号: MFCD00131849
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• リコンビナント: expressed in proprietary host
• 品質水準: 200
• フォーム: buffered aqueous glycerol solution
• 濃度: ≥900 DEA units/mL
• その他の活性: RNAse, DNAse, none detected
• 輸送温度: dry ice
• 保管温度: −20°C

詳細

詳細

Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15 minutes.

アプリケーション

Alkaline phosphatase was used to examine its role in the prevention of high-fat-diet-induced metabolic syndrome in mice. It also may be used in pasteurization process for milks used in dairy products.

生物化学的／生理学的作用

Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate linkages.

単位の定義

One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.

物理的形状

solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 1 mM MgCl₂

アナリシスノート

Protein determined by biuret

安全性情報

• ピクトグラム: GHS08
• シグナルワード: Danger
• 危険有害性情報: H334
• 注意書き: P261 - P284 - P501
• 危険有害性の分類: Resp. Sens. 1
• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable