48-690MAG

MILLIPLEX MAP T-Cell Receptor Signaling Magnetic Bead Kit 7-Plex - Cell Signaling Multiplex Assay

• 別名: 7-Plex Magnetic Bead Kit, Magnetic Bead Kit, T-Cell Receptor Signaling Kit
• UNSPSCコード: 12161503
• eCl@ss: 32161000
• NACRES: NA.47

特性

• 交差性: human
• 品質水準: 200
• メーカー／製品名: Milliplex^®
• テクニック: multiplexing: suitable
• assay range: inter-assay cv: 4-17%; intra-assay cv: 3-8%
• 検出方法: fluorometric (Luminex xMAP)
• 保管温度: 2-8°C

詳細

詳細

The Milliplex^® MAP 7-Plex Human T-Cell Receptor (TCR) Magnetic Bead Kit is used to detect changes in phosphorylated CREB (Ser133), Erk/MAP kinase 1/2 (Thr185/Tyr187), and phosphorylated tyrosine residues on CD3 epsilon chain, Lck, ZAP-70, LAT, and Syk in T-cell lysates using the Luminex^® system. Although the protein kinase Syk is not part of the T-Cell Receptor pathway, it does play a significant role in B-Cell Receptor signaling and acts as a negative control for the analysis of T-Cell Receptor signaling. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures.

特異性

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

アプリケーション

• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 μL diluted cell lysate.
• This kit must be run using Assay Buffer 2 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg cell lysate/well).

Research Category
Cell Signaling

This cell signaling panel includes the following analytes in this multiplex kit: CD3ε (pan Tyr), CREB (S133), ERK (Thr185/Tyr187), LAT (pan Tyr), Lck (pan Tyr), Syk (pan Tyr), ZAP-70 (pan Tyr).

Used to detect/quantify:CD3ε (pan Tyr)|CREB (S133)|ERK (Thr185/Tyr187)|LAT (pan Tyr)|Lck (pan Tyr)|Syk (pan Tyr)|ZAP-70 (pan Tyr)

保管および安定性

Recommended storage for kit components is 2 - 8°C

法的情報

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

安全性情報

• ピクトグラム: GHS05,GHS07,GHS09
• シグナルワード: Danger
• 危険有害性情報: H302,H315,H318,H410
• 注意書き: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• 危険有害性の分類: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
• 保管分類コード: 10 - Combustible liquids