C6079

コラーゲナーゼ + プロテアーゼインヒビター

Name: コラーゲナーゼ + プロテアーゼインヒビター
Brand: SIGMA

2-5 FALGPA units/mg solid, ≥800 CDU/mg solid

別名:

Collagenase preparation, Protease inhibitor solution

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About This Item

EC Number:

3.4.24.3 (BRENDA, IUBMB)

UNSPSCコード:

12352204

NACRES:

NA.54

主要文書

すべての文書を表示

テクニカルサービス

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テクニカルサービス

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由来生物

bacterial (Clostridium histolyticum)

品質水準

200

フォーム

solid

比活性

≥800 CDU/mg solid
2-5 FALGPA units/mg solid

分子量

68-130 kDa

溶解性

water: soluble

輸送温度

dry ice

保管温度

−20°C

遺伝子情報

rat ... LOC296948(296948)

アプリケーション

Collagenase with protease inhibitor has been used in a study to assess the association of immune system gene polymorphisms with quantitative features. It has also been used in a study to investigate the quantitative structure-activity relationship study on Clostridium histolyticum collagenase inhibitors. The enzyme from sigma has been used in the isolation of porcine pancreatic islets. It has also been used in the isolation of pancreatic-infiltrating lymphocytes from mice.

生物化学的／生理学的作用

Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four grams of calcium (Ca²⁺) per mole of the enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5 mg/mL are used for digestion. The duration of reaction can vary from 15 minutes to several hours for satisfactory cell dissociation without causing too much cell death. Zn²⁺ is required for activity. This product is used if the collagenase does not require a significant protease activity.