R8005

RPMI-1640 Medium

Hybri-Max^™, Modified, with L-glutamine, 4500 mg/L glucose and 15mM HEPES, without sodium bicarbonate, powder, suitable for hybridoma

• Synonym(s): RPMI 1640 Culture Medium
• MDL number: MFCD00217820
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• Quality Level: 500
• form: powder
• technique(s): cell culture | hybridoma: suitable
• impurities: endotoxin
• components: phenol red: 0.0053 g/L; L-glutamine: 0.3 g/L; HEPES: 3.5745 g/L; NaHCO₃: no; glucose: 4.5 g/L (Dextro)
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Quantity

Formulated to contain 16.4 grams of powder per liter of medium.

Reconstitution

Supplement with 2.0 g/L sodium bicarbonate.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable