R1137

Hind III from Haemophilus influenzae

Restriction Enzyme

• CAS Number: 81295-22-9
• EC Number: 3.1.21.4 (BRENDA, IUBMB)
• MDL number: MFCD00166570
• UNSPSC Code: 12352204

Properties

• grade: Molecular Biology
• form: buffered aqueous glycerol solution
• concentration: ≥10000 units/mL; 10,000 units/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

Specificity

Recognition sequence: 5′-A/AGCTT-3′
Cutting results: 2-10-fold Hind III overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 15 minutes

Application

HindIII, a restriction endonuclease, is used in molecular biology applications to cleave DNA at the recognition site 5′-A/AGCTT-3′ to generate DNA fragments with cohesive 5′-ends.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SB (B8781)

Comment: Hind III under suboptimal reaction conditions will cleave secondary recognition sites (star activity).

Physical form

Solution in 10 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 1 mM dithioerythritol, 250 mM NaCl, 0.01% polydocanol (v/v), 50% glycerol (v/v) at 4 °C

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable