P4306

Platelet-Derived Growth Factor-BB human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): PDGF-BB
• MDL number: MFCD00213023
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Product Name: Platelet-Derived Growth Factor-BB human, PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli
• Assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: 1.0-3.0 ng/mL EC₅₀
• quality: endotoxin tested
• mol wt: 24.3 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/μg
• UniProt accession no.: P01127
• storage temp.: −20°C
• Gene Information: human ... PDGFB(5155)

Description

General description

Platelet-Derived Growth Factor (PDGF), a cationic polypeptide, is an important serum mitogen predominantly found in cells of mesenchymal origin. PDGF is mapped to human chromosome 7p22.3 and is expressed in smooth muscle cells, endothelial cells and macrophages. PDGF exists as hetero and homodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD.

Application

Platelet-Derived Growth Factor-BB human has been used in induction of DNA synthesis and proliferation in low-concentration serum.

Biochem/physiol Actions

Platelet-derived growth factor (PDGF) is a potent chemoattractant and activator of neutrophils, monocytes, and fibroblasts. PDGF elicits multifunctional actions in a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells and chondrocytes. PDGF-BB isomer is highly expressed in tumor, mediating oncogenic pericyte recruitment and is is a key regulator of angiogenesis. PDGF-BB controls bone homeostasis, repair and regeneration. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins and modulates LDL receptor binding.

Other Notes

Dimer of the B chain of human PDGF.

Physical form

Lyophilized from a 0.2 μm filtered solution.

Analysis Note

The biological activity is measured by the dose-dependent stimulation of thymidine uptake in BALB/c 3T3 cells.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves