L5142

Lectin from Triticum vulgaris (wheat)

biotin conjugate, lyophilized powder

• Synonym(s): WGA, Wheat germ agglutinin
• UNSPSC Code: 12352202
• eCl@ss: 34058011
• NACRES: NA.32

Properties

• conjugate: biotin conjugate
• Quality Level: 200
• form: lyophilized powder
• composition: Protein, ≥80% E1%/280
• extent of labeling: 2-4 mol biotin per mol protein
• storage temp.: 2-8°C

Description

General description

Wheat germ lectin (WGA) from Triticum vulgaris exists as a homodimer and has multiple N-acetyl glucosamine (GlcNAc) binding sites. It is resistant to protein denaturation agents and is used for the detection of O-GlcNAc proteins. WGA binding to N-glycolylneuraminic acid (Neu5Ac) may modulate pro-inflammatory immune response.

Application

Lectin from Triticum vulgaris (wheat) has been used:

• in lectin binding assay of samples from intrauterine stages of filarial parasites
• in the histological studies of mice myocytes
• for the generation bead-biotin-WGA complex with Dynabeads^® M-280 Streptavidin magnetic beads for lectin magnetic separation of Toxoplasma gondii oocytes from water samples

Biochem/physiol Actions

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Packaging

Package size based on protein content.

Physical form

Contains sodium citrate

Preparation Note

Conjugates are prepared from affinity purified lectin.

Legal Information

Dynabeads is a registered trademark of Invitrogen Dynal AS Corp

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves