I3911

Protease Inhibitor Cocktail

Animal-Free, lyophilized powder, for the inhibition of serine, cysteine, aspartic and metalloproteases, for general use, lyophilized powder

• Synonym(s): protease inhibitor
• UNSPSC Code: 12352200
• NACRES: NA.77
• Form: lyophilized powder
• Solubility: water: soluble
• Storage temp.: −20°C

Properties

• Product Name: Protease Inhibitor Cocktail, Animal Component Free, for general use, lyophilized powder
• Quality Level: 200
• form: lyophilized powder
• solubility: water: soluble
• storage temp.: −20°C

Description

General description

Components of this cocktail are of non-animal origin and no animal products were used in the component production process. All vessels and instruments used for the cocktail production are dedicated for animal component free production and have never encountered an animal product. Aprotinin used in this cocktail is a recombinant bovine protein expressed in plants (Nicotiana).

This protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts. It contains inhibitors with a broad specificity for serine, cysteine, and acid-proteases, and aminopeptidases.

Specificity

Inhibits serine, cysteine, aspartic, and metalloproteases.

Application

Animal component free (ACF). Optimized and tested for general use. Protease Inhibitor Cocktail, Animal Component Free has been used to prevent protein lysis in saliva samples, peritoneal residential macrophage cells and in embryo culture medium.

Components

AEBSF
Aprotinin
Bestatin
E-64
EDTA
Leupeptin

Quantity

One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.

Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.

Physical form

Lyophilized powder that is water soluble.

The components of this ACF cocktail do not originate from animal sources and can thus be used in the production of animal free proteins.

Safety Information

• Pictograms: GHS05
• Signal Word: Danger
• Hazard Statements: H314
• Precautionary Statements: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Hazard Classifications: Eye Dam. 1 - Skin Corr. 1A
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable