G5893

Gamborg′s B-5 Basal Medium with Minimal Organics

fine powder, suitable for plant cell culture

• Synonym(s): B-5 Basal Medium, Basal Medium, Minimal Organics Medium
• UNSPSC Code: 12352207
• NACRES: NA.72

Properties

• form: fine powder
• Quality Level: 200
• technique(s): cell culture | plant: suitable
• application(s): agriculture
• storage temp.: 2-8°C

Description

General description

Gamborg′s B-5 basal medium is an efficient nutrient mixture for plants.

Gamborg′ B-5 medium may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro culture of plant cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis. Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.

Application

Gamborg′s B-5 Basal Medium with Minimal Organics has also been used:

• as a component of liquid media for the tissue culture of Brassica rapa
• as a component of callus inducing medium for culturing Arabidopsis cardiolipin mutant seedlings
• as a component of solid GM medium for germination of Arabidopsis seeds

Formula variant

With the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968).

Media Formulation

Quantity

Formulated to contain 3.2 grams of powder per liter of medium.

Safety Information

• Pictograms: GHS03
• Signal Word: Warning
• Hazard Statements: H272
• Precautionary Statements: P210 - P220 - P280 - P370 + P378 - P501
• Hazard Classifications: Ox. Sol. 3
• Storage Class Code: 5.1B - Oxidizing hazardous materials
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable