E6267

Earle′s Balanced Salts

With sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): EBSS
• MDL number: MFCD00217343
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• Quality Level: 400
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: NaHCO₃: 2.2 g/L; phenol red: 0.011 g/L; glucose: 1.0 g/L (Dextro)
• shipped in: ambient

Description

General description

Earle′s Balanced Salts is a balanced salt solution. It is used as an alternative for conventional simulated body fluids (c-SBF).

Recommended for suspension cultures or whenever cell clumping is a problem.

Application

Earle′s Balanced Salts has been used:

• to induce autophagy in mouse embryonic fibroblasts
• in the preparation of melanotroph isolation medium
• in the preparation of DNAse stock solution

Biochem/physiol Actions

Balanced Salt solution participates in providing cells with water, bulk inorganic ions, and nutrients like carbohydrates essential for normal cell metabolism. It acts as an irrigating, transporting, and diluting fluid to maintain cellular osmotic balance. Balance salt solution helps in maintaining the medium within the physiological pH range (7.2 to 7.6).

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable