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Merck

E4274

Sigma-Aldrich

Endotoxin Removal solution

Remove lipopolysaccharides (LPS) from plasmid DNA preparations

Synonym(s):

ET removal buffer, ET removal reagent, ET removal solution, endo removal buffer, endo removal reagent, endo removal solution, endotoxin contamination removal, endotoxin removal buffer, endotoxin removal reagent, plasmid purification endo, plasmid purification endo free

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.52

Key Documents

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Quality Level

200

Application

Endotoxin Removal solution has been used to remove lipopolysaccharide (LPS) from Baker′s yeast RNA.

Biochem/physiol Actions

The reagent relies on the micellar properties of Triton X-114 to remove endotoxins from solution. When warmed above 20 °C, micelles aggregate into a separate phase that sequesters amphiphilic solutes, including lipopolysaccharides. The hydrophilic DNA remains in the aqueous phase.

Quantity

1 volume treats 5 volumes of DNA solution (≤1mg/ml)

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

E4274-60ML-KC:
E4274-45ML-PW:
E4274PROC:
E4274-60ML-PW:
E4274-60ML:
E4274-25ML-PW:
E4274-BULK:
E4274-45ML:
E4274-VAR-PW:
E4274-25ML:
E4274-PH:
E4274-45ML-KC:
E4274-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCH4131
SLCF9991
SLBZ4931
SLBS0592V
SLBM1235V

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