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Merck

E2636

Sigma-Aldrich

ExtrAvidin® –Alkaline Phosphatase

buffered aqueous solution

Synonym(s):

Alkaline Phosphatase Reagent, ExtrAvidin Alkaline Phosphatase

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About This Item

MDL number:
MFCD00163600
UNSPSC Code:
12352203
NACRES:
NA.46

Key Documents

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conjugate

alkaline phosphatase conjugate

Quality Level

200

form

buffered aqueous solution

technique(s)

dot blot: 1:300,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections
indirect ELISA: 1:60,000

shipped in

ambient

storage temp.

2-8°C

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General description

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin has been conjugated to Alkaline Phosphatase and is used for various techniques.

Application

ExtrAvidin® -Alkaline Phosphatase has been used in:
  • Dot blot
  • Immunohistochemistry (formalin-fixed, paraffin-embedded sections)
  • Indirect ELISA
  • Enzyme linked immunosorbent assay (ELISA)
  • Enzyme-linked immunosorbent spot (ELISPOT) analysis
  • Competitive ELISA

Biochem/physiol Actions

Alkaline Phosphatase catalyzes the hydrolysis of esters to yield phosphoric acid. It is also facilitate the transport of substances across membranes.

Physical form

Solution in 0.05 M Tris-HCl buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin and 15 mM sodium azide

Preparation Note

Affinity purified protein

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

E2636-.2ML:
E2636-5X.5ML:
E2636-BULK:
E2636-.5ML:
E2636-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
088M4782V
21190319
109M4825V
079M4878V
21190318

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