CMC0012

XLDNA SIG10 Electrocompetent cells

Name: XLDNA SIG10 Electrocompetent cells
Brand: SIGMA

for BAC & cosmid cloning

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About This Item

UNSPSC Code:

12352200

NACRES:

NA.85

Key Documents

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biological source

Escherichia coli

grade

Molecular Biology

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: electrocompetent
transformation efficiency: ≥1 x 10¹⁰ cfu/μg

shipped in

dry ice

storage temp.

−70°C

General description

The XLDNA SIG10 Electrocompetent cells are designed for transformation of large constructs and increase in DNA yields.
These cells are manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts and are an excellent choice for large construct transformation

Genotype

F - pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 F80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupG λ- tonA (StrR)

Features and Benefits

Cells that enable the most challenging library construction and screening.
Highest available transformation efficiencies for library construction and screening.
Optimized for high yields of DNA

Components

XLDNA SIG10 electrocompetent cells
pUC 19 transformation control DNA
recovery medium for cloning

Storage Class Code

10 - Combustible liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.