93014

Manganese peroxidase from white-rot fungus (Phanerochaete chrysosporium)

powder, light brown, ≥10 U/g

• Synonym(s): Manganese-dependent lignin peroxidase, MnP, Peroxidase, manganese
• CAS Number: 114995-15-2
• EC Number: 1.11.1.13 (BRENDA, IUBMB)
• MDL number: MFCD03792385
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: fungus (white-rot fungus (Phanerochaete chrysosporium))
• form: powder
• specific activity: ≥10 U/g
• greener alternative product characteristics: Waste Prevention; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• color: light brown
• greener alternative category: Enabling,
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in fuel cell and cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research

Application

Manganese peroxidase from white-rot fungus (Phanerochaete chrysosporium) is from the peroxidase family and is used to oxidize manganese. It may be used to study wound healing.

Biochem/physiol Actions

Manganese peroxidase catalyzes the oxidation of Mn²⁺ to Mn³⁺. Mn³⁺ is then in complex with oxalate, which is able to oxidize lignin. Manganese peroxidase from Phanerochaete chrysosporium binds 2 calcium ions per subunit. It binds 1 heme B (iron-protoporphyrin IX) group per subunit .

Unit Definition

One unit corresponds to the amount of enzyme, which oxidizes 1 μmole Mn²⁺ per minute to Mn³⁺ at pH 4.5 and 25 °C

Physical form

Supplied as a lyophilized powder containing ~50% sodium tartrate

only partially soluble in water or buffer

Other Notes

This product is supplied as a mixture of isozymes, mol. mass (40-65 kDa).

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves