E7885

Elastase from porcine pancreas

lyophilized powder, suitable for cell culture

• Sinonimo/i: Elastase from hog pancreas, Pancreatopeptidase E
• CAS Number: 39445-21-1
• Numero CE: 3.4.21.36 (BRENDA, IUBMB)
• Numero CE: 254-453-6
• Numero MDL: MFCD00130998
• Codice UNSPSC: 12352204
• NACRES: NA.75

Proprietà

• Origine biologica: Porcine
• Livello qualitativo: 200
• Stato: lyophilized powder
• Attività specifica: ≥4 units/mg protein
• PM: 25.9 kDa
• Confezionamento: pkg of 1 μmol
• tecniche: cell culture | mammalian: suitable
• pH: 8
• Compatibilità: suitable for
• Attività estranea: Trypsin ≤50 BAEE units/mg protein
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.

Applicazioni

Elastase from porcine pancreas is used for the following applications:

• Cell culture techniques
• ELISA techniques
• Used in assay for elastase inhibitory activity

Azioni biochim/fisiol

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.

Enzyme that degrades elastin, a matrix component of tissues involved in stretching processes. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester.

Confezionamento

Package size based on protein content

Definizione di unità

One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.

Informazioni sulla sicurezza

• Pittogrammi: GHS08,GHS07
• Avvertenze: Danger
• Indicazioni di pericolo: H315,H319,H334,H335
• Consigli di prudenza: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classi di pericolo: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organi bersaglio: Respiratory system
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable