PPB005

Phosphate Buffered Saline

powder, pH 7.4, with 0.05% Tween^® 20, pHast Pack^™

• Sinonimo/i: PBS + 0.05% TWEEN^® 20, PBS with 0.05% Tween 20, PBS-T, PBST
• Codice UNSPSC: 12161700
• NACRES: NA.21

Proprietà

• Product Name: Tampone fosfato salino con 0,05% di TWEEN^® 20, pH 7,4, pHast Pack^™, powder
• Nome Commerciale: pHast Pack^™
• Stato: powder
• Condizioni di stoccaggio: (Tightly closed. Dry)
• Impurezze: ≤5 ppm Heavy Metals (lead)
• Colore: white
• pH: 7.2—7.6 (25 °C)
• Solubilità: soluble, clear to hazy, colorless
• Cationi in tracce: Fe: <10 ppm
• Attività estranea: DNase, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• Temperatura di conservazione: room temp

Descrizione

Descrizione generale

Phosphate-buffered saline with 0.05% Tween^® 20 or PBST contains PBS buffer with a low-concentration of Tween detergent. It is a common wash buffer and due to the presence of detergent, it can easily remove or block excess components thus reducing foaming and non-specific binding on the membrane and microtiter plate wells.

Applicazioni

PBST is used in research in a variety of assays including DNA or RNA hybridization assays and cell culture. It is commonly used for immunoassays like Western blot, ELISA, Dot blot, Immunohistochemistry (IHC), immunopercipitation (IP), and immunofluorecence. It has been used for incubation and wash buffer for peptide microarrays for performing High-density peptide array (HDPA) to analyze antibody responses against SARS-CoV-2.

Caratteristiche e vantaggi

• Ready-to-use buffer
• No measuring and pH adjusting needed
• Save time and minimize effort
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Confezionamento

Foil pouches

Ricostituzione

Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, 138 mM NaCl, 2.7 mM KCl; 0.05% Tween 20 (w/v), pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.

Stoccaggio e stabilità

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Altre note

For additional information on our range of Biochemicals, please complete this form.

Note legali

TWEEN is a registered trademark of Croda International PLC

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable