A8582

Antifoam SE-15

Name: Antifoam SE-15
Brand: SIGMA

aqueous emulsion for bacterial and mammalian systems

Synonym(s):

silicone-based antifoam

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About This Item

UNSPSC Code:

12161901

NACRES:

NA.85

Key Documents

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Quality Level

200

form

emulsion (aqueous)

concentration

10% (active silicon)

technique(s)

cell culture | hybridoma: suitable

solubility

H₂O: soluble (effective hot or cold.)

density

1.0 g/mL at 25 °C

application(s)

microbiology

storage temp.

room temp

suitability

(Mammalian (suspension))
bacteria (fermentation)

General description

Antifoam SE-15 is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.

Application

Antifoam SE-15 has been used:

in the preparation of ramified rolling circle amplification (RAM) amplification buffer.
to find its effects on cell viability, virus infectivity and PCR (polymerase chain reaction) performance.
as a constituent of phosphate-buffered saline (PBS) for the determination of total antioxidant status (TAOS) by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay.
as a component of autoinduction expression culture.
as a component of cyclic adenosine monophosphate (cAMP) detection buffer.