5006

Bovine Collagen Type I & III

from bovine hide, powder, suitable for cell culture, PureCol^™

• Synonym(s): Collagen Type I, PureCol Collagen
• EC Number: 232-697-4
• UNSPSC Code: 12352202
• NACRES: NA.75

Properties

• Product Name: PureCol^™, Collagen powder from Bovine hide, 97% Type I with the remainder of Type III collagen, suitable for cell culture
• biological source: bovine
• Quality Level: 100
• sterility: sterile-filtered
• Assay: 99.9%
• form: lyophilized
• packaging: pkg of 15 mg
• technique(s): cell culture | mammalian: suitable
• Binding Specificity: Peptide Source: Elastin; ; Peptide Source: Fibrinogen
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

PureCol^™ collagen is approximately 97% Type I with the remainder being comprised of Type III collagen. It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography.

It is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol^™, lyophilized form is not recommended for the formation of a solid gel. PureCol^™ collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.

Application

PureCol^™ has been used:

• in collagen gel preparation for the 3D sprouting assay with human umbilical vein endothelial cells (HUVEC) spheroids 
• to coat Costar^® Transwell^® Inserts plates before cell migration assays with bladder smooth muscle cells (BMSC)
• to coat culture plates for collagen degradation assay with breast cancer cell lines

Preparation Note

This product is supplied as a lyophilized powder with 15 mg of bovine collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

Legal Information

Costar is a registered trademark of Corning, Inc.

PureCol is a trademark of Advanced BioMatrix, Inc.

Transwell is a registered trademark of Corning, Inc.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable