SAE0219

Mucin Degradation Enzyme

for preparative digestions, powder

• UNSPSC kód: 41121800

Tulajdonságok

• Minőségi szint: 200
• Forma: powder
• eltarthatósági idő: 2 yr (retest)
• tárolási körülmény: protect from light
• kiszállítva: dry ice

Leírás

Általános leírás

Mucin Degradation Enzyme is a metalloprotease useful for digesting heavily O-glycosylated mucin proteins. This product is made for preparative scale digestions of viscous material that is dense with mucin-type glycoproteins.

Alkalmazás

For use as a sample preparation tool in research and discovery. Used for proteolytic degradation of mucin-dense samples to allow for downstream processing.

Biokémiai/fiziológiai hatások

The Mucin Degradation is based on a bacterial protease that specifically recognizes densely glycosylated mucins and mucin-domains, and proteolytically cleaves the peptide backbone. In nature this presumably enhances the virulence of the pathogenic microbe, but in the laboratory is a useful way of accessing molecular structures in tissues normally shielded by mucins.

Tulajdonságok és előnyök

• A promiscuous Mucin specific protease.
• Tool for viscosity reduction and tissue breakdown of mucin containing samples.
• Not inhibited by most general protease inhibitors such as AEBSF, Aprotinin, Bestatin, E-64, Pepstatin and Leupeptine. When needed digests can be done in presence of the ReadyShield^® Protease Inhibitor Cocktail PIC0002.
• Mucin Degradation Enzyme can be used to liberate soluble mucin fragments from cells or tissue without mechanical disruption or reduction.
• Mucin Degradation Enzyme can be used for enzymatic hydrolysis of mucins in the isolation of glyco-RNA samples.

Fizikai forma

Product is supplied as lyophilized powder containing the indicated amount of protein.

Elkészítési megjegyzés

Solution of Mucin Degradation Enzyme can be prepared by reconstitution of the lyophilized powder at a concentration of 1-5mg/ml protein. See CoA for lot specific percent protein in powder. The reconstituted protein solution may be clear or hazy and is stable for 2 weeks at 2-8 °C. For longer storage, aliquot the protein solution and store at -20 °C. The enzyme solution should be protected from direct light.

Tárolás és stabilitás

The product retains activity for at least 2 years when stored lyophilized at -20°C.

Egyéb megjegyzések

For applications that require more precision with highly purified enzyme like glycoproteomic experiments please consider using Mucinase StcE (SAE0202) and Mucinase SmE (SAE0220).

Jogi információk

Under licensing from Stanford University.

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Tárolási osztály kódja: 13 - Non Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable