EMS0004

SOLu-Trypsin

recombinant, expressed in Pichia pastoris, Proteomics Grade, liquid

• Szinonimák: Protein Digestion for Mass Spectrometry, Ready to Use Recombinant Trypsin for Protein Digestion in Mass Spectrometry, Recombinant Trypsin for Protein Digestion in Mass Spectrometry, Trypsin for Protein Digestion, rTrypsin
• UNSPSC kód: 12352204
• NACRES: NA.78

Tulajdonságok

• rekombináns: expressed in Pichia pastoris
• Minőségi szint: 200
• grade: Proteomics Grade
• leírás: Activity:≥ 10,000 unit/mg protein
• Forma: ready-to-use solution
• alkalmasság: suitable for mass spectrometry
• UniProt elérési szám: P00761
• kiszállítva: wet ice
• tárolási hőmérséklet: 2-8°C

Leírás

Általános leírás

Trypsin is a pancreatic serine endoprotease. SOLu-Trypsin is made from recombinant trypsin, porcine sequence. It does not possess chymotryptic activity.

Alkalmazás

Poster: Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays

SOLu-Trypsin:

• has been used to digest the gel pieces for mass spectrometry identification of active deubiquitnases (DUBs)
• has been used in trypsin sensitivity assay
• may be used for the digestion of wine proteins

Biokémiai/fiziológiai hatások

Trypsin is used in proteomics research for peptide mapping and protein sequence work due to its highly specific cleavage resulting in a limited number of tryptic peptides. It hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues.

• SOLu-Trypsin (EMS0004) is our exclusive, Advanced Proteomics Grade enzyme that is solution-stable for mass spectrometry.
• Designed to be stable in solution when refrigerated, SOLu-Trypsin can be used immediately without preparation.
• Other forms of trypsin require thawing or reconstitution, and must be discarded if not used immediately.
• SOLu-Trypsin allows excess product to be saved for future use, thus eliminating unnecessary waste and cost.
• It is formulated with a high-purity recombinant trypsin, free of chymotryptic activity, to ensure high fidelity digestion.

Tulajdonságok és előnyök

• Ready to use - no preparation, such as reconstitution or thawing, is required
• Fits seamlessly into established workflow - no need to modify protocols
• Eliminates waste - remains stable in the refrigerator after use so there is no need to discard excess product
• Recombinant, porcine sequence - no chymotryptic activity
• Stable for short-term use at room temperature in an autosampler or on a liquid handling robot

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable