T9285

Tris-EDTA buffer solution

100X concentrate for Northern and Southern blotting

• Szinonimák: Tris-EDTA wash buffer, TE buffer solution
• MDL-szám: MFCD00236359
• UNSPSC kód: 41105319
• PubChem Substance ID: 329826904
• NACRES: NA.25

Tulajdonságok

• grade: Molecular Biology
• Minőségi szint: 200
• sterilitás: 0.2 μm filtered
• Forma: liquid
• pH: 7.8-8.2 (1 × in water)
• alkalmazás(ok): sample preparation
• idegen aktivitás: DNase, RNase, none detected
• tárolási hőmérséklet: room temp
• SMILES string: NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
• Nemzetközi kémiai azonosító kulcs: VLEIUWBSEKKKFX-UHFFFAOYSA-N

Leírás

Általános leírás

Tris EDTA buffer is commonly used in molecular biology for reconstitution and storage of many precipitated biomolecules, including DNA and RNA. Tris-HCl is a commonly used buffer and EDTA is a metal chelating agent. TE buffer solubilizes nucleic acids while protecting the molecules from degradation. It is used in nucleic acid isolation, which may be done prior to nucleic acid gel electrophoresis and Northern or Southern blot hybridization.

Alkalmazás

TE buffer has been used for:

• isolate, resuspend, or store DNA and RNA for PCR or qPCR
• gel electrophoresis, including Northern and Southern blotting
• bacterial cells, for AmpC disk test, to measure β-lactamases released from the cells
• antigen retrieval for immunohistochemistry

Elkészítési megjegyzés

TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.

Egyéb megjegyzések

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Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: nwg
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable