M3817

MegaCell^™ RPMI-1640 Medium

without L-glutamine, liquid, sterile-filtered, suitable for cell culture

• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• Quality Level: 500
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: L-glutamine: no
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

MegaCell^™ is a line of versatile media, formulated to significantly reduce the amount of serum required for cultivating mammalian cells in vitro. It is effective as a basal formulation for the growth and maintenance of adherent or suspension cell lines. When supplemented with 3% fetal bovine serum (FBS), MegaCell supports proliferative rates and maximal cell densities comparable, and in some cases superior, to the conventional basal formulation supplemented with 10% fetal bovine serum.

Application

MegaCell^™ RPMI-1640 Medium has been used:

• as a component of the TSC medium for culturing trophoblast stem cells (TSC)
• as a component of 25 ng/ml FGF4 and 1 g/ml heparin (TSF4H medium) for culturing TSCs
• as a component of embryo-like structures (ETS) embryo medium and TS cell medium for culturing embryonic stem cells (ESCs) and TSCs

Reconstitution

Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513)

Legal Information

Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation.

MegaCell is a trademark of Promega Corp.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable