D8900

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

• Synonym(s): DMEM Hams F12, DME/F-12, 1:1 mixture
• MDL number: MFCD00217417
• UNSPSC Code: 12352207
• NACRES: NA.71

Properties

• Quality Level: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• components: sodium pyruvate: 0.055 g/L; HEPES: 15 mM; NaHCO₃: no; L-glutamine: yes; glucose: 3.15 g/L; phenol red: yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. It has been used:

• as a component of the culture medium for endometrial explants in vitro culture
• to immerse chitosan-gelatin-glycerol phosphate hydrogels for cytotoxicity evaluation
• to culture nucleus pulposus (NP) cells to culture human articular chondrocytes for evaluating the toxicity of baicalein

Quantity

Formulated to contain 15.6 grams of powder per liter of medium.

Reconstitution

Supplement with 1.2 g/L sodium bicarbonate.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1