S0149

Streptolysin O from Streptococcus pyogenes

γ-irradiated

• CAS Number: 98072-47-0
• EC Number: 308-500-3
• MDL number: MFCD00132389
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• biological source: Streptococcus pyogenes
• Quality Level: 200
• sterility: γ-irradiated
• form: lyophilized powder
• application(s): cell analysis
• storage temp.: 2-8°C
• Gene Information: Streptococcus pyogenes ... slo(900490)

Description

Application

Permeabilizes membranes to permit cellular uptake of large or charged molecules.

Streptolysin O from Streptococcus pyogenes has been used in streptolysin O (SLO) permeabilization. It has also been used to evaluate the barrier integrity and cytotoxicity of caco-2 and T84 IEC polarized monolayers.

Biochem/physiol Actions

Streptolysin O (SLO), obtained from Streptococcus pyogenes has the capability to block osteoclastogenesis, induced by receptor activator of nuclear factor κ-Β ligand (RANK) through the NF-κB signaling pathway. Streptococcus pyogenes (GAS) is a bacterial strain that results in bacterial arthritis, osteomyelitis and implant infections.

Thiol-activated toxin that permeabilizes animal cell membranes. The protein binds as a monomer to membrane cholesterol and subsequently polymerizes into large arc- and ring-shaped structures surrounding pores of >12 nm.

Thiol-activated toxin that permeabilizes animal cell membranes..

Unit Definition

One unit will cause 50% lysis of 2% red blood cell suspensions in phosphate buffered saline, pH 7.4, after incubation at 37 °C for 30 min.

Preparation Note

Prepared from S 5265

Safety Information

• Pictograms: GHS06
• Signal Word: Danger
• Hazard Statements: H300 + H310 + H330
• Precautionary Statements: P262 - P280 - P301 + P310 + P330 - P302 + P352 + P310 - P304 + P340 + P310
• Hazard Classifications: Acute Tox. 1 Dermal - Acute Tox. 1 Inhalation - Acute Tox. 1 Oral
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves