P7457

Carboxy-terminal FLAG-BAP^™ Fusion Protein

• MDL number: MFCD00130921
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• form: liquid
• Quality Level: 200
• mol wt: ~49 kDa
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Carboxy-terminal FLAG-BAP Fusion Protein is a 466 amino acid C-terminal FLAG fusion protein of E.coli bacterial alkaline phosphatase (BAP).

Application

Carboxy-terminal FLAG-BAP^™ Fusion Protein has been used in the immunoprecipitation of the reporter protein in human embryonic kidney (HEK) cell lysate and as a FLAG-tagged control protein in solid-phase binding assay of spermatogenic immunoglobulin superfamily protein (SgIGSF).

Learn more product details in our FLAG^® application portal.

Biochem/physiol Actions

The FLAG sequence comprises of the eight-amino acid sequence AspTyrLysAspAspAspAspLys and is hydrophilic. FLAG fusion proteins are expressed in bacterial, yeast and mammalian cells. FLAG epitope-tagged bacterial alkaline phosphatase is employed in immunoaffinity purification. Alkaline phosphatase based fusion protein have wide clinical applications in immunodetection, enzyme immunoassay and enzyme-linked immunosorbent assay.

Other Notes

Control protein

Physical form

Supplied in 10 mM Tris, 120 mM NaCl, 0.05 mM ZnCl2

Preparation Note

Dilute the ANTI-FLAG M2 antibody solution to 10 mg/ml

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves