G1041

EZBlue^™ Gel Staining Reagent

• Synonym(s): protein gel stain, protein stain
• UNSPSC Code: 41105300
• NACRES: NA.32

Properties

• technique(s): protein staining: suitable
• Quality Level: 200
• storage temp.: 2-8°C

Description

General description

Convenient, sensitive, and safe, EZBlue^™ Coomassie Brilliant Blue G-250 colloidal protein stain improves protein electrophoresis results while significantly reducing staining time. Conveniently packaged, EZBlue requires no messy weigh-ups or additions of methanol or acid. As a colloidal stain, it reacts only with proteins, not the gel itself. Background staining is reduced, so protein bands can be visualized almost immediately. No destaining step is required, although a water wash may intensify bands and clarify the background. Most impressively, EZBlue is extremely sensitive, detecting as little as 5 ng of protein.

Application

EZBlue^™ Gel Staining Reagent has been used as a staining reagent in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).

Features and Benefits

• Premixed solution eliminates the time and effort required to prepare the stain
• Increased sensitivity ensures that low abundance proteins can be detected (as little as 5 ng)
• Rapid reaction significantly reduces the amount of time required to stain and rinse
• No solvent waste so you save time and money by eliminating hazardous material disposal

Other Notes

A ready-to-use Brilliant Blue G-250 based protein stain for "one step" ultrasensitive detection on polyacrylamide gels and PVDF membranes.

Legal Information

EZBlue is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Pictograms: GHS08,GHS05
• Signal Word: Warning
• Hazard Statements: H290,H315,H319,H371
• Precautionary Statements: P234 - P260 - P264 - P302 + P352 - P305 + P351 + P338 - P308 + P311
• Hazard Classifications: Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2
• Target Organs: Eyes,Central nervous system
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves