T8280

Tris Acetate-EDTA buffer

10× concentrate, BioReagent, Molecular Biology, DNase and RNase, none detected, powder blend, suitable for electrophoresis

• Sinónimos: TAE buffer
• Número MDL: MFCD00236357
• Código UNSPSC: 41105319
• ID de la sustancia en PubChem: 329826878
• NACRES: NA.25

Propiedades

• grado: Molecular Biology
• Nivel de calidad: 200
• Línea del producto: BioReagent
• Formulario: powder blend
• impurezas: DNase and RNase, none detected
• pH: 8.1-8.5
• idoneidad: suitable for electrophoresis; suitable for gel electrophoresis (after dilution to working concentration)
• aplicaciones: diagnostic assay manufacturing
• cadena SMILES: CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)
• Clave InChI: HGEVZDLYZYVYHD-UHFFFAOYSA-N

Descripción

Aplicación

Ready for use in gel electrophoresis after dilution to working concentrations.

TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Envase

Packaged in plastic bottles large enough to contain the concentrate after reconstitution.

Nota de preparación

Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).

Reconstitución

Produces a 10× concentrate (0.4 M Tris-acetate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Gloves