T6025

Tris Acetate-EDTA buffer

BioReagent, suitable for electrophoresis

• Sinónimos: TAE buffer
• Número MDL: MFCD00236357
• Código UNSPSC: 41105319
• ID de la sustancia en PubChem: 329826837
• NACRES: NA.25

Propiedades

• esterilidad: 0.2 μm filtered
• Nivel de calidad: 200
• Línea del producto: BioReagent
• Formulario: working solution
• técnicas: electrophoresis: suitable
• impurezas: DNase, RNase, Protease, none detected
• idoneidad: suitable for electrophoresis; suitable for gel electrophoresis (after dilution to working concentration)
• aplicaciones: diagnostic assay manufacturing
• cadena SMILES: CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)
• Clave InChI: HGEVZDLYZYVYHD-UHFFFAOYSA-N

Descripción

Aplicación

TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Tris Acetate-EDTA buffer has been used as run buffer for the agarose gel electrophoresis of human papillomavirus DNA and transcribed RNA samples.

Otras notas

40 mM Tris acetate, pH approx. 8.3, containing 1 mM EDTA.

Nota de preparación

Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).

Solution prepared with 18 megohm water

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)