F1283
Fetal Bovine Serum
Fetal Bovine Serum, USA origin, Ultra Low IgG
Sinónimos:
FBS, FCS, Fetal Calf Serum
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About This Item
Código UNSPSC:
12352207
NACRES:
NA.71
Servicio técnico
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Permítanos ayudarleServicio técnico
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Permítanos ayudarleorigen biológico
bovine fetus
Nivel de calidad
esterilidad
sterile-filtered
Línea del producto
FBS Select
composición
Bovine IgG, <5 μg/mL
Hemoglobin, ≤20 mg/dL
origen
USA origin
condiciones de almacenamiento
avoid repeated freeze/thaw cycles
protect from light
técnicas
cell culture | mammalian: suitable
impurezas
≤10 EU/mL endotoxin
pH
6.8-8.1
osmolalidad
260-330 mOsm/kg H2O
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
Descripción general
Animal serum is commonly used to supplement basal media formulations for the optimal growth of many cell types in vitro.
Fetal Bovine Serum (FBS) is the most common serum used to supplement cell culture media due to its high nutritional content. Although it is relatively low in protein, FBS is effective in promoting and sustaining growth of vertebrate mammalian and insect cells.
IgG is the most common immunoglobulin present in FBS. Elevated levels of IgG may cause problems within certain recombinant protein or monoclonal antibody purification processes. For applications where IgG may interfere or cause issues, we have depleted IgG levels in this fetal bovine serum by sequential chromatography down to a level of < 5 ug/mL, while maintaining performance equivalent to regular FBS.
Fetal Bovine Serum (FBS) is the most common serum used to supplement cell culture media due to its high nutritional content. Although it is relatively low in protein, FBS is effective in promoting and sustaining growth of vertebrate mammalian and insect cells.
IgG is the most common immunoglobulin present in FBS. Elevated levels of IgG may cause problems within certain recombinant protein or monoclonal antibody purification processes. For applications where IgG may interfere or cause issues, we have depleted IgG levels in this fetal bovine serum by sequential chromatography down to a level of < 5 ug/mL, while maintaining performance equivalent to regular FBS.
Envase
Packaged in sterilized, graduated plastic bottles and sealed with a tamper indicator. Bottles are immediately frozen and stored at -10 to -40 C.
Nota de preparación
The fetal blood is aseptically collected and allowed to clot under controlled conditions. After centrifugation, the raw (unfiltered) serum is decanted from the clot, pooled and immediately frozen.
Frozen raw serum is thawed under controlled conditions and then processed through a series of membrane filters in descending pore size. FBS is filtered through three 0.1 μm filters before packaging.
Frozen raw serum is thawed under controlled conditions and then processed through a series of membrane filters in descending pore size. FBS is filtered through three 0.1 μm filters before packaging.
Nota de análisis
Endotoxin and hemoglobin tested
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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